Recombinant Kex2 Serine Protease Expressed in Pichia pastoris Purified By HPLC

Recombinant Kex2 Serine Protease Expressed in Pichia pastoris Purified By HPLC

Recpmbinant Kex2 Protease, yaxinbio

1.DESCRIPTION

Kex2 is a Ca2+ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF,TPCK,TLCK inhibition.The optimal pH of Kex2 protease is 9.0. It is stable in buffer (pH 5.0-6.0).

2.MAIN FEATURES

Source: Pichia pastoris

Appearance: White or off white powder

Specific activity: ≥10.0 units/mg pro.

Purity(SDS-PAGE): Single major band

Molecular Weight(SDS-PAGE): 67.0±6.7kDa

UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.

3.RECOMMEND USAGE

Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca2 + or HEPES, 5 mM Ca2 +. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2 + buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 + or HEPES, 5 mM Ca2 + buffer.

Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage:Recombinant kex2 protease should be stored under 2-8℃ in sealed container.

It is stable within 6 months.After dissolved with 20mM NaAc-HAc,pH 5.2,2mM Ca2+,it should be stored under -20℃.

Stability of transport:The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

Animal origin free:Recombinant Kex2 protease is no exogenous virus contamination,and any animal origin material is not used in

the production process.

Stable quality: Mass production can ensure stable and continuous batch production.There is no difference between the batch and the

product quality is stable.

High purity: Higher specific activity and less host protein residues than the limits of biological products.

Lyophilized powder: The form of product is lyophilized powder and it is easy to store and transport.

Compliance with regulatory requirements: Producing equipment and environment comply with relevant regulatory

requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.