Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified

Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified

Recombinant Kex2 Protease

Main Features

Source: Pichia pastoris

Specific activity: ≥10u/mg

12.5%SDS-PAGE: Single main stripe

Mol.weight: 67±6.7 kDa

Recommend storage buffer:pH5.0-5.5 20mM NaAc-HAc buffer and 2mM Ca²⁺.

Recommend reaction buffer:pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca²⁺ .

To prepare 1-10mg/ml Kex2 solution with 20mM NaAc-HAc (pH 5.2) and 2mM Ca²⁺ for a stored solution, and diluted with reaction buffer shch as pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca²⁺. If used after dissolved immediately, with the reaction buffer pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca²⁺ to dissolve directly.

DESCRIPTION

Kex2 is a Ca²⁺ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF,TPCK,TLCK inhibition.The optimal pH of Kex2 protease is 9.0. It is stable in buffer (pH 5.0-6.0).

PRODUCT ADVANTAGE

Animal origin free:Recombinant Kex2 protease is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

Product Infomation

Product Specific Recombinant Kex2 Protease

activity ≥10u/mg

Packaging 50µg/each

Manufacturer YaxinBio